Slip melting point determination for the quality control of lipstick or palm stearin

m565Measuring the slip melting point (SMP) is a popular and important method used for the quality control of waxy compounds in the cosmetic or food industries.

Such applications can be easily performed using the standard BUCHI M-565 Melting Point instrument. The obtained results show a high reproducibility and the process can be automatically recorded for later analysis if required.

Two application notes clearly demonstrate that SMP measurements can be reliably performed for the quality control of lipstick or palm stearin following the USP recommendations.

Buchi NIR, Extraction and Kjeldahl Solutions for Dairy Products

Effective quality control is essential in the dairy products industry. The healthy image that these products enjoy, especially the perception of their safety and quality, is always under close scrutiny due to occasional highly publicized product recalls involving milk, cheese, ice cream, and related products.

Buchi is a leading global supplier of dedicated solutions for the milk and dairy industry. Our extensive experience within the industry involves both classical reference methods and innovative FT-NIR technology for highly reliable quality control of milk and dairy products.

Dairy products analysis with Buchi NIRMaster™:
Preparation time: <1 min.
Analysis time: 30 sec. – 2 min. for liquid milk
Materials required: NIRMaster, unbreakable cup, and specific calibration
Dairy calibrations available for: raw milk, milk powder, WPC, butter, cream, buttermilk, ice cream, yogurt, chocolate
More NIR calibrations are available.

Protein determination analysis using automated Kjeldahl:
Instruments required: Digestion units SpeedDigester K-425/436/439 and Scrubber B-414, Kjelflex K-360 with external titrator from either – Mettler, Metrohm, Schott, or Radiometer or Buchi Kjeldahl Sampler system K-370/371
Preparation time: 2 – 3 min./sample
Analysis time: Digestion: 60 min., Distillation/titration: 5 – 6 min.
Available applications: see the complete listing of Kjeldahl Applications

Fat determination analysis using automated hot extraction:
Instruments required: Hydrolysis unit E-416 and Hot Extraction unit E-816
Preparation time: 2 – 3 min./sample
Analysis time: Acid hydrolysis: 45 min., Drying (microwave): 30 min., Extraction solvent recovery: > 45 min., Drying to constant weight: 20 min.

Dairy Product Analysis

NIR, Extraction & Kjeldahl Analysis on Dairy Products

Separation of Plant Extracts Using Sepacore Flash Chromatography System

Natural Plant ExtractsNatural compounds are more and more evaluated as alternatives to classical drugs and therefore the needs for the separation of such complex mixtures are also growing. Flash chromatography is a very valuable technique in the field of natural compounds research because it provides a fast and economical way to separate the main components of complex plant extracts. Download this flash chromatography study and view our flash chromatography applications for other related studies.

Introduction:
During the last decade, several systems for rapid preparative chromatography with pre-packed cartridges have been commercialized. Pre-packed cartridges ensure rapid separation cycles and ease of use.

Flash chromatography systems were initially developed for rapid and easy purification of synthetic products, and numerous applications are documented in the experimental section of publications in synthetic chemistry. In contrast, application of such systems in the separation of complex natural product mixtures such as extracts has been neglected.

The aim of this work was to explore the potential and limitations of cartridges for the purification of natural product extracts. Empirical rules have been established for the determination of the separation conditions by preliminary TLC and HPLC analyses.

The influence of solid introduction compared to liquid injection has been also investigated. The performance of the cartridges was compared to that of classical selfpacked MPLC glass columns for the separation of complex plant extracts of medicinal importance.

Instrumentation:
Preparative separations were performed on a Sepacore® chromatography system (Büchi Labortechnik) consisting of two C 605 pump modules, a C 620 control unit, a C 635 UV detector and a C 660 fraction collector. The system was controlled by the software SepacoreControl 1.0.

Flash chromatography separations were performed on pre-packed silica gel (40 – 63 μm) and RP18ec (40 – 63 μm) polypropylene cartridges (12 x 150 or 40 x 150 mm, Büchi) at a flow rate of 10 mL (12 x 150 mm cartridges) and 30 mL/min (40 x 150 mm cartridges), resp. Medium pressure liquid chromatography (MPLC) separations were carried out on a glass column (26 x 460 mm) packed with silica gel Si60 (15 – 40 μm) or LiChroprep RP18 (25 – 40 μm) (Merck), at a flow rate of 10 mL/min. Liquid injection was carried out through a 6-way valve with a 20 ml loop. Solid introduction was performed by means of a PrepElut cartridge (Flash chromatography) or a glass precolumn (MPLC) connected to the top of the cartridge or the column, resp. The samples were adsorbed to silica gel Si60 or LiChroprep RP-18, resp., prior to introduction.

Conclusions:
• Reversed phase HPLC separations can be transposed by increasing the gradient time
by a factor 2-4.

• For normal phase separations, solvent compositions resulting in Rf values of 0.15-0.2 on TLC for the most lipophilic and the most hydrophilic constituents, respectively, should be selected as gradient endpoints.

• Sepacore® cartridges enabled a good separation of compounds with a broad range of polarity, as typically found in plant extracts. The chromatographic resolution remained, however, lower than that achieved by MPLC on columns packed with material of smaller particle size. For poorly soluble extracts, solid introduction gave better results than liquid injection.

• Despite lower resolution as compared to MPLC, pre-packed cartridges are an attractive alternative for the purification of extracts and crude fractions due to their ease of use and speed of separation.

NIRMaster’s Benefits for Food Safety & Quality in Milk & Dairy Production

Buchi NIRMaster - FT-NIR Spectrometer

Buchi NIRMaster - FT-NIR Spectrometer

Food safety and consistent quality are essential in milk and dairy production. Accurate and meaningful nutritional facts are important. Understanding customer needs in the milk and dairy industry, BUCHI has designed the first standalone FT-NIR Spectrometer specifically for that industry.

The NIRMaster™ is the first dedicated FT-NIR standalone spectrometer. The NIRmaster is an integral part of BUCHI’s larger NIRSolutions product line providing dedicated at-line and off-line NIR laboratory equipment improve your business.

BUCHI has developed a brochure specifically for the Milk & Dairy industry. Request this NIR Brochure and talk to one of our specialists with any questions or feedback.

Some of the benefits include:

1. The hygienic easy-clean design and high ingress protection (IP) support your routine work in harsh environments. The NIRMaster™ is available in food-grade PMMA housing or stainless steel housing.

2. The modern and robust NIRMaster™ based on fast and precise polarization FT-NIR technology, provides accurate and reliable results for multiple parameters simultaneously.

3. The NIRMaster™ with its built in PC, preinstalled software and quick-start calibrations can be quickly set up and put into operation.

4. The NIRMaster™ is supported by a twin-lamp safeguard for uninterrupted operation even in cases of lamp failure. Based on robust and accurate polarization technology it is more shock resistant than any other FT-NIR.

5. In cooperation with many of BUCHI’s long-term customers we have concentrated data and experience in our pre-calibrations. These powerful quick-start calibrations enable you to efficiently analyze a broad range of your sample constituents.

6. BUCHI NIR spectrometers are identical by design. Spectra and calibrations can be exchanged between laboratory and production units without any calibration standardization. Furthermore, your existing calibrations, even data from other suppliers, can be transferred easily to your new BUCHI FT-NIR spectrometer.

7. Use the powerful NIRCal software to evaluate your production data. Keep an eye on what is going on at your sites. For evaluating or updating a calibration NIRCal is the ideal tool. The patented Calibration Wizard and the comprehensive NIRCal Toolbox support even unexperienced users building up powerful calibrations.

NIR Brochure: Advantages of NIRMaster for Feed & Forage Industry

Buchi Near Infrared Solutions

Buchi Near Infrared Solutions

Production of compound feeds is a complex matter. Accurate and meaningful nutritional facts are most important. Understanding customer needs in the feed and forage industry, BUCHI has designed the first standalone FT-NIR Spectrometer specifically for that industry – the NIRMaster™.

Please contact Buchi and request our new NIR segment brochure highlighting the major quality and production control advantages of the NIRMaster FT-NIR Spectrometer for the Feed & Forage Industry.

Get more analysis information, higher productivity, higher quality and better understanding in feed production. Rapidly control the quality and nutritional facts of your feed ingredients, pre-mix, and final products using BUCHI NIRSolutions. The NIRMaster™ is the first dedicated FT-NIR standalone spectrometer. Its easyclean design and high ingress protection (IP) meet the specific needs for at-line analysis in compound feed production.

Many different constituents of compound feed such as moisture (dry matter), fat, protein, ash, NDF, ADF, amino acids, starch, total sugar, crude fiber and many more can be qualified and quantified.